LOCUS       40924_23464       11712 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB617 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11712)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11712)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2921..2950
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2957..2986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3002..3031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3038..3067
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3098..3350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3384..3400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3408..3424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3432..3462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3477..3498)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3534..3786)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3949..4497)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4549..4728)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5234..5258)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5779..6567
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6816..7404
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7590..7730)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8074..8268)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8337..9407)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9839..10465)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacggt gaacaaaagc taatctccga
     2941 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg
     3001 tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga
     3061 agacttgaac ggtagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     3121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     3181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     3241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     3301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt
     3361 tccggaacca attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3421 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3481 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag
     3541 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3601 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3661 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3721 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3781 acgatcgggg aaattcgagc tggggacgtc cccagctcgg taccccctcc tgggcacggt
     3841 cgtacgcgat ccgccgcggt tccacctcac cattgcgggt atgccagttg agttcgatga
     3901 gttcccgtac cagctccaac tcgggcgatg ccacgcgcac tccgttcaga tctcggtgac
     3961 gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc
     4021 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
     4081 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
     4141 gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt
     4201 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
     4261 tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
     4321 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
     4381 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
     4441 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatatc
     4501 ttattgcccc ccgggatcag atctggattg agagtgaata tgagactcta attggatacc
     4561 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     4621 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     4681 taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa
     4741 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     4801 cgctcatgat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     4861 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     4921 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     4981 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     5041 gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     5101 gcgccctgag tgcttgcggc agcgtgaagc tgatccgtcg agattaatag aaataaattc
     5161 agcctaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     5221 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     5281 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     5341 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     5401 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     5461 attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca
     5521 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     5581 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     5641 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     5701 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     5761 aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca
     5821 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     5881 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     5941 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     6001 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     6061 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     6121 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     6181 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     6241 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     6301 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     6361 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6421 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6481 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6541 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg
     6601 ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag
     6661 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     6721 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
     6781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6961 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7441 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7501 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7561 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7621 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7681 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     7741 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7801 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7861 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
     7921 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
     7981 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
     8041 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
     8101 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
     8161 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
     8221 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
     8281 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
     8341 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
     8401 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
     8461 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
     8521 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
     8581 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
     8641 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
     8701 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
     8761 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
     8821 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
     8881 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
     8941 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
     9001 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
     9061 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
     9121 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
     9181 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
     9241 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
     9301 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
     9361 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
     9421 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc
     9481 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
     9541 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
     9601 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
     9661 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
     9721 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
     9781 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
     9841 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
     9901 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
     9961 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
    10021 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
    10081 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
    10141 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    10201 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    10261 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    10321 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    10381 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    10441 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    10501 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    10561 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    10621 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    10681 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    10741 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    10801 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    10861 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    10921 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    10981 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    11041 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    11101 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    11161 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    11221 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    11281 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    11341 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    11401 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    11461 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    11521 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    11581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    11641 atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    11701 acggataaac ct
//
